Type: Optics / Microscopy,
Keywords: 3D-Neural imaging, Miniature fiber-coupled two photon microscope, Electrowetting lens technology
2P-FCM: Miniature 2-photon fiber-coupled microscope for 3D neural imaging in freely moving animals
We are developing a two-photon miniature fiber-coupled microscope that uses electrowetting lens technology for three dimensional neural imaging in freely moving animals. We are currently working to disseminate the technology to five beta users for testing in different animal models.
* 2P-FCM is a light-weight fiber-coupled head-mounted miniature two-photon microscope (<2 grams) for imaging in awake freely moving mice.
* Employs electrowetting lenses for high-speed variable focusing with no moving parts in a compact design.
* Allows rapid imaging in multiple focal planes and tilted focal planes.
* Uses include imaging neural activity in animals performing complex behavior tasks such as navigating odor plumes or social interactions.
* Mice (Thy1-GCaMP6s, C57BL/6 mouse)
* Prairie voles
* Provides optical sectioned imaging with tunable depth of 200 microns for 3-dimensional imaging.
* Extremely light weight (<2 grams).
* Ability to do fast multiplane imaging and tilted field imaging.
* Lateral resolution is limited to ~ 1.8 microns due to the pixelation from the imaging fiber bundle, although this resolution can still show neural activity from cell soma and processes.
* The 2P-FCM can be added on to any existing two-photon microscope.
Baris N. Ozbay et al. 2018, Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning. Scientific Reports 8(1):8108. doi: https://www.nature.com/articles/s41598-018-26326-3
Rocky Mountain Summit on Wearable Miniature Microscopes: https://www.cuneurophotonics.org/conference.html
Emily Gibson, Associate Professor
University of Colorado Denver, Anschutz Medical Campus
TEAM / COLLABORATOR(S)
Victor Bright, Professor, University of Colorado Boulder
Juliet Gopinath, Associate Professor, University of Colorado Boulder
Diego Restrepo, Professor, University of Colorado Anschutz Medical Campus
Karl Kilborn, Co-President, Intelligent Imaging Innovations, Inc. (collaborator)