sciMAP-ATAC
Posted on October 26th, 2020
single-cell combinatorial indexing on Microbiopsies Assigned to Positions for the Assay for Transposase Accessible Chromatin (sciMAP-ATAC). High-throughput single cell genomic assays resolve the heterogeneity of cell states in complex tissues, however, the spatial orientation within the network of interconnected cells is lost. We present a novel method for capturing spatially-resolved epigenomic profiles of single cells within intact tissue, and apply this method to generate non-neuronal cell taxonomy atlases of human and mouse cortex. This method will be made accessible through protocols.io and all data, along with single cell analyses, will be made available through the BRAIN Initiative Cell Census Network (BICCN)
CaMPARI & Voltron
Posted on October 26th, 2020
CaMPARI (Calcium Modulated Photoactivatable Ratiometric Integrator) – a fluorescent protein-based integrator of calcium for permanent marking of neuronal activity, Voltron – a chemigenetic fluorescent voltage indicator for in vivo recording of electrical activity. Primary use cases are marking/monitoring of neuronal activity in vivo in model organisms. Goal is to disseminate as broadly as possible, primarily via publicly-accessible repositories, to enable new biology.
MAPseq & BARseq
Posted on October 25th, 2020
BARseq and MAPseq can map long-range projections of 1000s of neurons in a single brain area at single neuron resolution, and further correlate projections to gene expression and Cre. We achieve mapping of densely labeled neurons at single neuron resolution by cellular barcoding and sequencing. Our methods allow comparison of projections across neuronal subtypes within an animal, across individual animals, and across genotypes. We offer MAPseq service through CSHL core facility, and may offer BARseq service in the future. We also welcome other labs to adopt both methods on their own.